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To determine the etiology, we used the multiplex PCR strategy based on the Resplex assays described below, and performed additional singleplex PCR assays to determine the influenza subtype.
Furthermore, the rapid PCR strategy for snapback primer assay is highly dependent on the air flowing PCR instrument [ 40].
To mimic the natural mechanism providing loop variability in antibodies, we developed an overlap PCR strategy.
Exon-primed intron-crossing (EPIC) PCR strategy employs conserved exon sequences to design universal primers (Lessa 1992).
In this study, a long PCR strategy was used to rapidly genotype for the C4A deletion through specific primer design.
Following a successful validation, the multiplex PCR strategy was implemented in the routine testing of MRSA for national surveillance.
Colony PCR strategy was performed to screen the pET28b-kIspS-C-term in BL21 (DE3) using the kIspS specific primers with the same amplification conditions as mentioned previously.
To obtain the genome segment terminal sequences, a two-step PCR strategy was used.
The point mutations were obtained using an overlapping PCR strategy described in detail elsewhere [64].
All hANT knock-in DNA fragments were constructed using the same PCR strategy described above.
Point mutations in TAS1Rs were made using the same overlapping PCR strategy with mutagenic primers.
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