Sentence examples for pcr scheme from inspiring English sources

For any discrepant PCR results, we re-confirmed serotype by quellung reaction and re-adjusted the PCR scheme to achieve optimal conditions to resolve these discrepancies.

Study isolates and one CC118/2 isolate were blind-tested with the new alternative PCR scheme; all were correctly clustered in groups of the corresponding CC.

The multiplex PCR scheme established in this study is a simple, rapid and reliable method for the identification of the B. fragilis group species.

PFA comprises software for sequence design, data management, and the generation of instruction sets for liquid-handling robotics, a liquid-handling robot, a robust PCR scheme for gene assembly from synthetic oligonucleotides, and a genetic selection system to enrich correctly assembled full-length synthetic ORFs.

The 1st reaction of the multiplex PCR scheme (Figure 2) reported here could detect the serotype of up to 40% of invasive pneumococcal cases.

The sequential multiplex PCR scheme (Figures 2 and 3), as expected, identified the capsular serotype of isolates in decreasing order of frequency.

PCR schemes were as follows: 94°C for 3 minutes; 94°C for one minute, 45°C (p7, p9), 50°C (p8, p10, rp49) for one minute, 72°C for one minute, 36 cycles; 72°C for 10 minutes.

We isolated this genomic region by two sets of PCR schemes.

PCR schemes were developed to detect IPD associated serotypes distribution [ 21, 23- 26].

However, as serotypes associated with carriage and with diseases differ, the proposed PCR schemes were not optimal to rapidly serotype colonizing pneumococci.

The urge of a simple multiplex-PCR scheme to specify these clones has motivated the present study.