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To avoid PCR and sequencing errors, two independent PCR samples for each ecotype were separately sequenced.
The validity of the PCR-RFLP analysis was confirmed by direct sequencing of several PCR samples with each genotype.
PCR samples were preheated for ten min at 95°C and then subjected to 35 PCR cycles.
For $599, you'll get all the parts to the machine, instructions to set it up, and 16 PCR samples — the way by which you target certain regions of the DNA.
Error bars represent standard derivation from duplicated PCR samples of the same donor.
Error bars represent standard derivation from duplicated PCR samples of same donor.
Similar(10)
RT-PCR samples were then treated with and without the indicated restriction enzymes obtained from New England Biolabs.
Equivalent RNA loading was routinely assessed in RT-PCR samples by monitoring GAPDH and LaminA/C housekeeper mRNA levels with primers as described [81].
To prepare RNA for qRT-PCR, samples were precipitated in 100% ethanol, washed twice with 70% ethanol, then resuspended in autoclaved double-distilled water.
The lower band present in IL-24 RT-PCR samples corresponds to a splice variant, which has been described before [41].
This may explain the failure of isolation from the three positive RT-PCR samples.
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