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RNA was amplified by using the reverse transcription PCR reported by Couacy-Hymann et al. (9 ).
Borrelia species were first identified by using the nested PCR reported by Ras et al. (4 ).
The presence of the PPR viral RNA was determined in samples by using a specific reverse transcription PCR reported by Polci et al. (8 ).
Previous studies based on serologic tests, culture, immunohistochemistry, and polymerase chain reaction (PCR) reported conflicting results concerning the role of Borrelia in the pathogenesis of LS.
Frye and colleagues assessed the impact of real-time PCR, reporting on timely identification of clustered Gram-positive cocci in BCs and on the appropriate choice of antimicrobial treatment [ 14].
Our results showed that levels of SUV39H1 protein were not significantly different between SLE CD4+ T cells and healthy controls, consistent with the mRNA levels detected by real-time PCR reported in our previous study [ 21].
This was confirmed by a positive N-PCR report of his aqueous specimen.
The etiological diagnosis was confirmed by positive N-PCR (Figure 3) and RT-PCR report of aqueous specimen (Additional file 1).
Together, promoter activities assessed by reporter genes and transcript levels determined by quantitative RT-PCR reported qualitatively similar results on the salt-dependent upregulation of CNGC19 and CNGC20 in leaves of mature plants.
agr types were determined using two sets of M-PCRs reported previously [8].
In accordance with aDNA guidelines, all DNA extractions and PCRs reported in this study were performed in a separate and dedicated aDNA laboratory at Murdoch University (Perth, Australia).
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