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This combined PCR process required approximately 200 PCR reactions pairing each of the primers.
Although PCR process is labor-intensive and time consuming, PCR can theoretically produce one million copies from a single double-stranded DNA molecule after 30 cycles.
A validation via PCR and Sanger-sequencing can also not be performed as the competitive PCR process would confer an advantage the more common variant.
The PCR process can be treated as a problem in biochemical engineering.
This method required a PCR process of 38 40 cycles and ordinary PCR reagents.
Then the ligated DNA probe acted as polony template that was amplified by PCR process in the thin polyacrylamide hydrogel.
The reactive groups involved must be sufficiently stable to tolerate the high temperature applied in the PCR process.
A set of specific primers of polymerase chain reaction (PCR) process were designed to control the selectivity of specific DNA detection.
The accuracy of the detection of hgDNA584 and hgDNA528 was controlled through the design of specific primers in the PCR process.
In the PCR process, specifically designed tagged-primers are used, i.e. a forward primer consisting of a tag domain, which can conjugate with gold nanocolloids on the chip, and a primer domain, which can anneal/amplify the target sequence.
The PCR process consists of three steps: denaturation of target DNA, annealing of sequence-specific oligonucleotide primers and the enzyme-catalyzed elongation of the annealed complex (primer:DNA:polymerase).
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