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The direct PCR procedure for each regenerated clone was described previously (Yamagishi et al. 2013).
After optimization, the PCR procedure for sex determination was applied to 14 embryo biopsies.
The PCR procedure used for ssDNA shuffling is basically carried out in two steps.
The PCR procedure was designed according to the instruction manual of a GeneRacer Kit (Invitrogen, Carlsbad, CA, USA).
To optimize PCR at various annealing temperatures, we evaluated each primer pair using a gradient PCR procedure.
In order to identify and quantify this bacterium in "morcilla de Burgos", a new specific PCR procedure has been developed.
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RT-PCR procedure and primer sequences are in SI Materials and Methods.
A nested-PCR procedure for the DNA amplification of those phages was developed.
The sequences of the primer pair and the multiplex RT-PCR procedure were described previously in Huang et al. (2012).
The aim of this study was to develop an RT-PCR procedure to determine proPO gene expression.
A real-time-PCR procedure was introduced in 2002 with similar sensitivity, but a more rapid response.
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