Sentence examples for pcr phase from inspiring English sources

The RT step was performed at 50°C for 30 minutes, and inactivated at 95°C for 30 seconds, the PCR phase consisted of one cycle at 95°C for 15 minutes followed by 50 cycles with three steps, of 94°C for 15 seconds, 58°C for 30 seconds and 72°C for 1 minute.

In the second PCR phase, the annealing temperature is decreased to allow the core region of the NLS primers to hybridize to the enriched target sequence.

PCR cycle numbers were optimized separately for cv-lysozyme 3 and 28 S rRNA to ensure the reactions were terminated within the linear non-saturated PCR phase.

RT- DraI-PCRs were assembled as normal RT-PCRs, but an initial PCR phase (3 cycles) was used to produce double-stranded cDNA.

random hexamers, but instead by the sequence-specific reverse primers themselves – thereby limiting reverse primer presence to unknown extents during the final (PCR) phase of each target amplification reaction.

The thermal profile was as follows: RT phase consisted of 5 min at 42 °C followed by 10 s at 95 °C; and PCR phase of 40 cycles of 5 s at 95 °C and 31 s at 60 °C.

The prevalence increased as detection technology changed from cell culture (phase I), to RT-PCR (phase II), to real-time RT-PCR (phase III).

The test involves DNA extraction, multiplex polymerase chain reaction (PCR), solid phase reverse hybridization and detection of the resistance mutations [ 18- 20].

In this article, we compare the normalized fluorescence data point of the two fluorescence channels during the early PCR exponential phase, and furthermore describe a rational real time data point analysis strategy for quantifying allele ratios in DNA pools.

To avoid using Ct values in quantification allele frequency, a novel rational data processing method, which is based on the normalized fluorescence ratio Kf of real-time PCR exponential phase, was presented.

The RT-PCR exponential phase was determined on 18 28 cycles, to allow semi-quantitative comparisons among complementary DNAs (cDNAs) developed from identical reactions.