Sentence examples for pcr mixed from inspiring English sources

A complete PCR mixed was prepared (20 μl per reaction) by combining an equal volume of PCR primer mix (Dxterity) and 2X Dynamo probe master mix (New England Biolabs, F-450 L).

For genomic PCR, mixed stage worms were treated with proteinase K and used directly as template.

For RT-PCR, mixed stage worms were washed from nearly starved plates and RNA was prepared using TRIzol (Invitrogen).

Stratagene SYBR Green PCR master mix or Applied Biosystems qRT-PCR master mixed was used for RT-PCR or PCR as needed.

Of these methods only PCR on mixed colonies from 4-day-old cultures on 4% hoof agar media yielded results of practical value.

To obtain microbial genomic DNA for PCR amplification, mixed or clonal microbial cultures were pelleted by centrifugation and the resulting pellet was resuspended in lysis solution using Genomic DNA Extraction kit (Axygen Scientific Inc., Tewksbury, MA, USA) and extracted according to the manufacturer's instruction.

Because chimeric DNA sequences can be obtained when PCR amplifying mixed, degraded DNA sources, all sequences unable to be identified to a low taxonomic level were analysed with chimera detection software (Ccode; [38]) against other identified sequences from the same clone library.

It is common for PCR on mixed templates to lead to recombination events during the reaction [ 38, 39].

The products of these first two PCR were mixed and a sequential PCR was performed with the bgal15_START_F and bgal15_STOP_R primers.

The four fragments of each PCR were mixed in equimolar proportion and subject to high-coverage paired-end sequencing in a MiSeq Illumina machine.

The detection method employed a modified E6/E7-specific consensus PCR, using mixed primers (pU-1M, pU-1M-L/pU-2R and pU-2R-N).