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AMK developed the multiplex PCR method.
Colony PCR method is more widely used than plasmid PCR method in confirming the gene insertion into a vector.
The Papuga PCr method is the best solution at present.
Site-specific mutagenesis was performed with a simple PCR method.
So, we accepted the separated allele-specific PCR method.
(B) Schematic presentation of the separated allele-specific PCR method for designing the SPIKE-01SNP marker.
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Therefore, the AS-PCR method includes two independent PCR reactions.
This LETAIL-PCR method also adopted the reported TAIL-PCR method (Liu and Chen 2007).
The mu-IFN-CSP gene was constructed by improved SOE-PCR method.
Open image in new window Fig. 4 EC 50/IC 50 measured by qRT-PCR method.
In brief, the S89G mutation was introduced in exon 6 using an overlap extension-PCR method.
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