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Bisulfite-converted DNA (40 ng) was amplified by PCR using the PyroMark PCR Kit (Qiagen, Valencia, CA, USA).
The first PCR was done using the Expand long template PCR kit, with 5min.
Quantitative RT-PCR reactions were performed using SYBR green fluorescent quantitation PCR kit.
The real-time PCR kit was purchased from GeneCopoeia.
Subsequently, qRT-PCR was measured using miScript SYBR Green PCR Kit (QIGEN, Shanghai).
Viral load was measured in the supernatant by a commercial quantitative PCR kit.
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Reverse-transcription PCR (RT-PCR) was performed using OneStep RT-PCR kit, and bands resolved on a 2% agarose gel and visualised with GelRed dye (Biotium, CA, USA).
RT-PCR reactions were performed using the Qiagen OneStep RT-PCR Kit (Qiagen, Valencia, CA).
Adapters also supplied in the SQK-PCS108 cDNA-PCR kit were then ligated to the PCR product (Supplementary Fig. 1).
RT-PCR was performed using a One Step SYBR PrimeScript PLUS RT-PCR Kit (Takara Bio Inc.).
The MinION sequencing library was prepared using the SQK-PCS108 cDNA-PCR kit (Oxford Nanopore Technologies Ltd).
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