Sentence examples for pcr it from inspiring English sources

Therefore, considering the advantages of LAMP over PCR, it can be used in most of molecular methods that utilize PCR.

Given the extreme sensitivity of PCR, it is now commonplace to detect and quantify transcripts present in extremely low abundance.

By using oligonucleotide primers (Pm0762F/R and Pm1231F/R) designed from these genes in PCR, it was found that specific DNA products of expected sizes were obtained with genomic DNA from P. multocida only, but not from other bacteria.

Finally, when the method trueness of the DNA-based sensing system was evaluated by monitoring real samples (fresh cockles) with the referenced polymerase chain reaction (PCR), it was able to successfully distinguish the available V. parahaemolyticus in the treated group (spiked and unspiked) samples.

Since microarray applications comprise highly multiplexed assays that are run under only one condition (similar to multiplex PCR), it is desirable to equalize probe characteristics such as Tm and ΔG.

Because this method uses linker-mediated PCR, it can be adapted for the small-scale analysis of developing germ cells.

It is important to note that if the in vitro synthesized RNA is to be used for RT-PCR, it must be free of any precursor plasmid which can function effectively as template for the PCR.

Using real time trans-PCR, it was possible to obtain a clear positive reading for 8.01 × 103 cells/ml.

As it can be combined with other advanced genetic detection methods (e.g., single-cell RT-PCR), it pushes the development of an automated platform for CTC-based cellular and molecular assays.

Gel-MIH was expressed only in the EG, as determined by RT-PCR; it was not detected in Y-organ, heart, integument, gill, testis, ovary, hepatopancreas, thoracic ganglion, or skeletal muscle.

Using AS-PCR, it was evident that the levels of murine mtDNA decreased throughout preimplantation development up to the blastocyst stage but at ever-decreasing levels (P<0.001).