Sentence examples for pcr extension from inspiring English sources

Approximately 28.356 kb of foreign gene sequence from CT-4 cosmid and by further PCR extension reaction was obtained.

Approaches to mitigate primer interaction and eliminate inhibitors were tested, including: reduction of primer concentrations especially those with preferential amplification; decrease of PCR extension temperature; increase of extension time and PCR cycles; and addition of bovine serum albumin.

The Fd fragment was further extended to a complete Ab heavy chain by SOE PCR extension with primers PH1 CH1 F SOE, CH1 Hi B, CH2 F, CH2 B, CH3 F, and CH3 B, using pBRhIG1 as template for the hinge, CH2, and CH3 regions.

A cloning cassette was synthesized by PCR extension of the GFP fragments.

PCR extension times of 6 minutes did not produce larger cDNA fragments.

PCR extension times of 2 minutes yielded cDNA fragments of approximately 1 kb or less (Figure 1A).

Post-PCR extension analyses of sequencing oligonucleotides mapping immediately upstream each SNDM allowed us to quantify using MALDI-MS the proportion of PCR products derived from the CNV region versus the external reference.

Using RACE-PCR extension approach, complete CDSs were obtained for 28 ERF unigenes that are representative of the main ERF sub-groups.

The PCR program consisted of preheating at 98°C for 10 sec, 30 cycles of 98°C for 10 sec, 55°C for 30 sec, and 72°C for 5 min, and post-PCR extension at 72°C for 10 min. The relative abundance of each specific 5'UTR and 3'UTR primer-defined P450 transcript was measured using Real-time Quantitative PCR as described above.

A crtS expression cassette was constructed by OE-PCR (overlap extension- PCR) and cloned into plasmid pBluescript SK-, resulting in the plasmid pBS-PTEF crtS-Tact.

PCR and extension primers were pooled and balanced according to the MassARRAY protocol (Agena Bioscience, San Diego, CA, USA).