Sentence examples for pcr composition from inspiring English sources

The PCR composition includes 1.25 U of Taq DNA polymerase, 15 mM Tris HCl, 50 mM KCl, 1.5 mM MgCl2, 0.1% BSA, each dNTPs at a concentration of 200 μM, 0.25 μM of each primer, and 60 100 ng of template DNA.

PCR composition and thermal cycling were used as described previously (Dietrich et al. 2012).

PCR buffer composition may play a role in the performance of an EG master mix.

The PCR mixture composition and procedure were the same as that of scrAB, but the annealing temperature was 64°C.

To provide a basis to the divergent chitinase activity levels across habitats (singling out the bacterial contribution), direct molecular analyses of the microbiota in term of abundance (real-time PCR) and composition (PCR-DGGE) were the next steps applied.

The PCR mix composition was the same as described above for liver abscess pus PCR, except that 1.0 μl of 25 mM MgCl2 was added.

The PCR mix composition was the same as described above for liver abscess pus PCR, except that 1.0 μl of 25 mM MgCl2 and 2.5 μl of template DNA was added.

The PCR reaction composition using PyroMark PCR Kit (QIAGEN #978703, QIAGEN, Inc., Valencia, CA, USA) are HotStarTaq DNA Polymerase, 1× PyroMark PCR Buffer, dNTPs, 1× CoralLoad, 3 mM MgCl2, 0.2 uM Primers, 1× Q-solution, and 20 ng bisulfate-converted DNA.

Six different protocols to isolate DNA from a wine and three PCR mix compositions were tested, and the best method was selected.

Primers were combined into groups to reduce the number of PCR reactions (the composition of primer groups is provided in Table 2.

In addition to the 2 μl template from the first round PCR, the reaction composition of the second round PCR was 10 μl 10× Titanium Taq PCR Buffer, 8 μl dNTPs (2.5 mM each), 1 μl Titanium Taq, 5 μl FwdGEX Primer (10 μM), 5 μl RevGEX Primer (10 μM) adjusted to a total of 100 μl with PCR-grade water.