Sentence examples for pcr combines from inspiring English sources
Multiplex-ready PCR combines the principles of the M13-tailed primer method [ 10] and two-step multiplex PCR amplification [ 11, 20].
Molecular diagnosis exploiting PCR combines several advantages; it is minimally invasive, has a high sensitivity and specificity, is capable of identifying relapses and reinfections in treated VL patients, and can provide species identification [5], [6].
In this sense, multiplex-ready PCR combines both the advantages of the M13-tailed primer method and multiplex PCR for fluorescence-based SSR genotyping.
The method called Universal RT microRNA PCR combines the benefits of a universal RT reaction with the specificity of two miRNA-specific PCR primers [ 15].
This method, termed multiplex-ready PCR, combines the advantages of the M13-tailed primer method and multiplex PCR in a single step, closed tube assay.
The mRNA transcription levels of gadB2 gene in recombinant C. glutamicum during fermentation were determined by real-time PCR (RT-PCR) combined with reverse transcription as described previously (Wang et al. 2015).
These results showed that infectivity PCR combined with the glass-beads-based DNA extraction method was useful for the detection of RCA in adenovirus vector products.
In this study, the diversity of Aeromonas spp. present in environmental samples was assessed by a PCR combined with DGGE approach.
The multiplex PCR combined with CE method was applied to test 50 patients, and all of the test results were compared with the DNA sequencing method, long-PCR method and real-time PCR method.
To assess the medical applicability of CpG methylation as molecular markers for cancer diagnosis, we established a new system to determine DNA methylation based on TaqMan PCR combined with a methyl-binding-domain polypeptide 2.
Furthermore, gene polymorphism was found by performing a PCR-RFLP (PCR combined with restriction fragment length polymorphism) on both the 102 bp and the entire ema-1 gene DNA amplified from two B. equi isolates, Florida (USA) and Pelotas (Southern Brazil) isolates.
