Sentence examples for pcr combinations from inspiring English sources

This allowed us to confirm our positive results through the use of two PCR combinations.

For identification and characterisation of homozygous insertion mutants, genomic DNA isolated from appropriate wild type and mutant plants was used for PCR analysis, using various PCR combinations and primers.

The nested PCR combination of C05 and C97 [13] followed by a reverse complement of primer C98 [13] and1067r [14] was selected as it yielded a final product of suitable length (∼400 bp) for sequence analysis.

The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp internal fragment of the gene, which was finally characterised by inverse PCR as the first full-length sequenced serine protease gene (nasp) from Dermatophilus congolensis.

Each of the three HCMV PCR primer combinations showed satisfactory performance.

The PCR primer combinations for each gene were as follows: VvCCD4a (XM_002268368): forward 5'-CCACACAGCCTTCACTCTCA-3', reverse 5'-AGGGCCTTTTTGAGAAGCAT-3'.

To obtain 5'-end coding sequences for the Acdp genes, we conducted a series nested PCR with combinations of mouse and human primers.

That this single type was found from multiple different PCR primer combinations with varying conditions also argues against it being a PCR artifact caused by polymerase slippage between poly-T regions.

PCR primers, combinations used, and sequences are shown in table 2. Modern hair and blood DNA were prepared in a separate laboratory and never brought to laboratories conducting aDNA work so as to avoid potential contamination.

Prior to the RAP-PCR, different combinations of reverse transcription and PCR primers were tested to achieve the combinations that gave the best span in fragment sizes.

The only published data for galectin-3 in urine is a combined assay of galectin-3 methylation specific PCR in combination with GSTP1 (Glutathione S-transferase P1) promoter methylation.