For identification and characterisation of homozygous insertion mutants, genomic DNA isolated from appropriate wild type and mutant plants was used for PCR analysis, using various PCR combinations and primers.
This allowed us to confirm our positive results through the use of two PCR combinations.
The only published data for galectin-3 in urine is a combined assay of galectin-3 methylation specific PCR in combination with GSTP1 (Glutathione S-transferase P1) promoter methylation.
Importantly, Lasso was far less computationally burdensome than MLR and PCR in combination with tabu search.
Error prone PCR in combination with recombination of beneficial mutations resulted in a variant with increased specific activity for 2-phenylpropionitrile at pH 7.5.
The modified PCR, in combination with an improved DNAzol™-based DNA extraction technique, was very specific and sensitive (detecting between 5 and 72 R. salmoninarum cfu per mg head kidney tissue).
The PCR primer combination consists of a forward GSP positioned in the internal sequence and the M13 primer.
This suggests a higher detection threshold of the msp1F3 PCR in combination with a lower influence of fluctuations in parasite density on detectability.
HLA typing by NGS has been demonstrated by performing long range PCR in combination with shotgun sequencing [ 3- 5].
Using the genogroup specific oligonucleotide probes used in this monoplex GI and GII real-time PCR the combination in a multiplex PCR resulted in ineffective detection.
