Sentence examples for pcr cocktail from inspiring English sources

To avoid contamination, sterile filter-tips were used, and sample preparation, DNA extraction, PCR cocktail preparation, PCR amplification and gel electrophoresis were performed in four different laboratories with unidirectional sample movement.

To prevent contamination DNA extraction, PCR cocktail preparation, amplification, and analysis of results were carried out in physically separated rooms.

The residence time of the PCR cocktail in each temperature zone decreased and the transition times between zones increased at higher PCR cocktail flow velocities, leading to less time for the amplification reactions.

For the small footprint reactor array, 0.4 mm wide and 1.2 mm high fins were necessary within the groove to cool the PCR cocktail efficiently, with a temperature gradient of 15.8 °C/mm, as it flowed from the denaturation zone to the renaturation zone.

The PCR cocktail was performed as previously described during 30 cycles.

A single master PCR cocktail containing all reagents other than the genomic DNA and barcoded SSU338R [32] primers was set up then aliquoted into a 96-well PCR microtitre plate.

Following the initial screening performed of eight individuals, we developed four multiplex PCR cocktails containing a total 10 polymorphic loci for large-scale genotyping (Table S1).

Polymerase chain reaction (PCR) cocktails and DNA sequencing reactions were carried out as previously described [ 53].

DNA extraction employed a glass-fibre protocol [ 72], while polymerase chain reactions (PCR) were performed using standard PCR cocktails [ 70].

Polymerase chain reaction (PCR) cocktails and DNA sequencing reactions were carried out as previously described [ 22, 23].

Amplification of fragments was accomplished in 50 ul reactions and PCR cocktails were identical to those of Ardren et al. [ 49] with approximately 50 100 ng of G. flavescens genomic DNA.