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The AMP PCR is a PCR based technique that we applied to study DNA methylation profiles in different cell types.
Site-directed mutagenesis was performed using PCR based technique to replace required amino acids [29].
As a targeted polymerase chain reaction (PCR) based technique that largely avoids non-specificity, FGF may be a viable and cost-effective alternative to array and solution based hybridization enrichment technologies.
McKinney et al. also used a quantitative PCR based technique, but the assay used may not be specific for FCGR3B, due to the presence of FCGR3A SNPs (rs57263253 and rs60966737) in one of the TaqMan primers [55].
Contrary to this, PCR based technique can detect even a few bacteria.
IL-10 gene polymorphisms were determined by sequence-specific oligonucleotide probing using a standard PCR based technique.
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In this work we compare two competing enrichment protocols, an emulsion-PCR based technique from RainDance technologies [ 18] and a method utilizing solution-based RNA-bait hybrid capture marketed as the SureSelect Target Enrichment Platform [ 19].
The presence of amplifiable DNA was confirmed for all 129 cases by PCR based-technique using primers for a fragment of β-globin gene and therefore all DNA samples were adequate for further analysis.
Different methods have been applied to Y. pestis for this purpose, such as PFGE(pulsed-field gel electrophoresis)[8], [9], MLST(multilocus sequence typing)[4], VNTR variable number of tandem repeat)[10], [11], [12], Ribotyping[13], RAPD randomly amplified polymorphism DNA)[14], [15], IS (insertion sequence) based typing [4], [16], and PCR-based technique as well[17].
TPH allelic variants were determined in each subject using a PCR-based technique.
Gβ3 allelic variants were determined in each subject using a PCR-based technique.
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