Sentence examples for pcr based strategy from inspiring English sources

A PCR based strategy using degenerate phr-specific primers was designed to detect and analyze possible photolyase genes.

Site-directed mutagenesis was performed using a PCR based strategy.

As a complementary approach, we isolated endothelial cells from Pkd1endo− (n = 5) and Pkd2endo− (n = 4) embryos as well as controls and assayed for deletion using either a PCR based strategy or Western analysis (Figure 7E, F, G, H).

Sequence verified riboprobe generation templates corresponding to bps 884 1238 of Gapdh (Accession # NM_001001303) and bps 294 705, 792 1185 and 294 1185 of Il7 (Acc # NM_008371) were produced via a PCR based strategy that utilized forward primers flanked by the T3 promoter and reverse primers flanked by the T7 promoter.

A PCR based strategy was devised to amplify the four different transcripts from the subunit A gene.

We generated point mutations using a standard PCR based strategy and verified the identities of individual clones through double stranded plasmid sequencing.

A database of germline immunoglobulin sequences was used to design 112 primers for a nested (RT- PCR based stRT- PCRto cover all VH, VL, JH, JL, CH and CL gene families/genes from C57basedand BALB/c mice.

These predicted exons were then tested for expression using an RT-PCR based strategy.

A PCR-based strategy using degenerate primers based on the amino acid sequence of carp granulin-1 led to the cloning of two zebrafish cDNAs sharing 92.3% identity and encoding structures homologous to carp granulin-1 and granulin-2, respectively (see Materials and Methods, Figure 2 and Additional File 1).

The rearranged variable heavy and light chain antibody regions were amplified from hybridoma cDNA by RT-PCR and recombined into one PCR product using a fusion PCR-based strategy that, after cloning into a suitable expression vector, enables the expression of a recombinant antibody from a single expression vector, as described [10].

However, all these studies were based on data sets obtained by retrieving chromosomal targeting sequences from a mixed population of transposon targeted cells or using a PCR-based strategy.