Sentence examples for pcr based mutagenesis from inspiring English sources

We used inverse PCR based mutagenesis [30] to generate all mutants.

Constructs that encode mutants of Tat were generated by PCR based mutagenesis.

Dvl2-K446M mutant was generated by a PCR based mutagenesis approach.

These vector templates were used for generation of CMG mutants through PCR based mutagenesis.

The serine at amino acid position 65 was changed to threonine by PCR based mutagenesis.

In this assay, we use PCR based mutagenesis to produce T7 templates including the mutations of interest, and then use these DNAs to produce the mutant RNA templates.

Deletions, point mutations and epitope insertions were generated by PCR-based mutagenesis.

GFP-BFL1ΔC (lacking the C-terminal 24 residues) was generated by PCR-based mutagenesis using the Quickchange mutagenesis kit (Stratagene).

Mutant DNAs were generated by PCR-based mutagenesis using the Quickchange mutagenesis kit (Stratagene), or were purchased at GenTech.

Second, by including PCR as an integral step in the procedure, they make PCR-based mutagenesis strategies convenient.

The short and compact His6-tag was placed on the previously constructed expression vector pCANTAB 5 E that contained the large affinity E-tag sequence (13 amino acids) by PCR-based mutagenesis and was expressed in Escherichia coli.