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A fusion PCR based method was employed for the construction of gene knockout fragments [ 64].
Point mutations (Fig. 3A) were introduced into construct −232/+36 using a PCR based method.
To prevent genotyping errors DNA samples were genotyped in duplicate by RealTime PCR based method.
Firstly, the MRC1 ORF was substituted with KanMX6, with a PCR based method [27].
The detection of the HPV was performed using a real-time PCR based method previously described [ 13].
A quantitative PCR based method targeting the M1 influenza gene was developed to quantify pharyngeal viral load.
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In this paper, we propose a principal-component-regression- (PCR-) based method for estimating RT variability.
The levels of the NIS mRNA were assessed by a semi-quantitative RT-PCR based method, as previously described [ 12].
GSTM1 and GSTT1 null alleles were identified using a multiplex-polymerase chain reaction- (PCR-) based method [ 15].
It is worth mentioning that down-regulated VvWRKYs under cold-treatment were only identified by qRT-PCR based method.
5S rDNA and U2 snRNA probes were labeled by the PCR-based method using PCR primers as previously mentioned.
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