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The hybridizations localized the breakpoints at sufficiently high resolution to enable PCR based breakpoint cloning.
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The smaller deletions were confirmed by PCR-based breakpoint mapping methods.
Primers for RT-PCR were chosen based upon published breakpoint sequences.
PCR amplification is based on nested-PCR.
Split-read methods detect breakpoints based on a read spanning a breakpoint and, as a result, length of the sequence read as well as the sequence complexity of the breakpoint determine sensitivity (Karakoc et al., 2011).
A segmentation algorithm computes the new coordinates of the breakpoint based on the alignments.
Gene mutations were analyzed using high-resolution microarray and validated by quantitative PCR with breakpoint sequencing.
MICs are based on EUCAST breakpoints.
†Interpretation based on Clinical and Laboratory Standards Institute breakpoints (M100-S23) (11 ).
Susceptibility interpretations for other antimicrobial drugs were based also on breakpoints established by NCCLS (24).
Moreover, genotyping by sequencing facilitates increased precision to determine recombination breakpoint compared to previously constructed genetic linkages maps based on sparse PCR-based markers (Huang et al. 2009; Spindel et al. 2013).
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