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The third PCR based approach was designed for phylogenetical analysis.
M35-1 was isolated using a PCR based approach by designing specific primers.
The replication timing was assessed using a PCR based approach [22], [46].
PCR based approach was also used to analyze for the correct integration of the hyg and neo based replacement constructs.
We employed a PCR based approach to monitor recombination between repeats.
A PCR based approach was taken to evaluate transcript levels for the four different subunit A mRNAs.
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In this study we present data describing the viability and limitations of a pooled-PCR based approach to GS20 sequencing.
We chose to use a miRNome real-time RT-PCR based approach instead of microarray for several reasons.
This was a result of the small quantities of RNA that can be obtained from biopsies, which required the use the RT-PCR based approach.
Using an RT-PCR based approach, we have shown that at least 8 different mutant transcripts are produced from the T Wis allele.
Unfortunately the spectrum of sequences obtained through PCR-based approach is limited by the specificity of the PCR primers used.
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