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First, Sanger DNA sequencing of PCR amplifier was performed.
PCR Amplifier was produced by Eppendorf Company (Hamburg, Germany).
The PCR amplification was performed in a 7500 real-time PCR amplifier (ABI, USA) under the following conditions: 95°C for 30 s, 40 cycles of 95°C for 5 s, and 60°C for 34 s.
Expression levels of AR, MIP-1α, MIP-1β, MIP-2 and IL-10 were determined by quantitative real-time PCR using iCycler real-time PCR amplifier (Bio-Rad Laboratories).
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Q-PCR reactions and analysis were performed with GoTaq Q-PCR MA6002 Mix (A6002, Promega) on a Bio-rad CFX96 Q-PCR Amplifier.
Semi-nested PCR was used to acquire the amplifier.
PCR was carried out on a Tercik DNA amplifier (DNA Technology, Russia) by using DreamTaq PCR Master Mix (Fermentas, EU), under the following conditions: 1 cycle at 94°C for 10 min, 25 cycles at 94°C for 45 sec, 54°C for 45 sec, 68°C for 2 min, 1 cycle at 68°C for 7 min, and a final soak step at 4°C.
The emulsion PCR was carried out in a SOLiD EZ Bead Amplifier (Life Technologies, Carlsbad, USA) using the E80sm setting.
PCR for 16S 23S rRNA gene cluster was carried out on a Tercik DNA amplifier (DNA Technology, Russia) by using DreamTaq PCR Master Mix (Fermentas, EU), under the following conditions: 1 cycle at 94°C for 10 min, 25 cycles at 94°C for 45 sec, 54°C for 45 sec, 68°C for 2 min, 1 cycle at 68°C for 7 min, and a final soak step at 4°C.
NinjaPCR is the name of a DNA amplifier that is supposed to cut down the costs associated with polymerase chain reaction (PCR), the process of cloning DNA in vitro, by up to 90%.
I play amplifier".
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