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48 h later, the HBB site of each embryo was PCR amplified individually.
* Indicates that target fragments in 5 GFP+ embryos failed to be PCR amplified.
The region spanning the target site was then PCR amplified, subcloned into TA vectors, and sequenced.
Androgen Receptor gene was PCR amplified as an internal positive control.
The polymerase chain reaction (PCR) amplified products were subjected to DNA sequencing.
The regions spanning the gRNA target sites were then PCR amplified for the T7E1 assay.
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RT-PCR amplified preferentially HevPBANR-C from female pheromone glands.
The PCR- amplified products of 24 isolates were sequenced.
They used a PCR-amplified genomic clone of cv.
(B) Gel electrophoresis of PCR-amplified GUS fragment.
The PCR-amplified DNA fragment, corresponding to FLO5, was digested with Asci I and ligated to Asci I digested PCR-amplified pEPG fragment.
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