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Herman, J.G., Graff, J.R., Myohanen, S., Nelkin, B.D. & Baylin, S.B. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.
After PCR, a thermal melt profile was performed to examine the homogeneity of the PCR application.
Another test for Lyme disease involves PCR, a technique that allows scientists to amplify the number of copies of a specific region of DNA.
Using PCR, a scientist can take a single DNA molecule and copy it many times, making it easier to read and to manipulate.
Kato, K. Adaptor-tagged competitive PCR: a novel method for measuring relative gene expression.
After PCR, a DpnI digestion is performed, which cleaves only the methylated template plasmid DNA.
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After PCR, A-tailing and ligation into pTargeT vector (Promega) was performed.
This provided the binding site for the phosphorylated primer PCR-A.
Six hours later, expression of Hamp1 mRNA from livers was measured by qRT-PCR (a), and the hepcidin concentration in the serum was measured by ELISA (b).
Another popular variation is reverse transcription polymerase chain reaction (RT-PCR), a technique used to detect and measure RNA.
To obtain highly accurate and reliable results in a real-time RT-PCR a highly defined calibration curve is needed.
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