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The PCR 1 reactions for each replicate and time point were combined and used directly to optimize conditions for PCR 2. PCR mixes were essentially similar to PCR 1, but contained 1 µL of pooled PCR 1 products as template, and 0.15 µM each of forward and reverse primers (Supplementary Data 13).
The final protocol used for PCR 1 for the GeCKO libraries is described below.
Cycling conditions were optimized for each PCR 1 product using Q5 polymerase.
The detection limit of the AO-LAMP assay was 10 fg, exceptionally higher than the conventional PCR (1 pg).
By infectivity PCR, 1 pfu of RCA spiked into 109 particles of adenovirus vectors could be detected.
In the ideal case, the X-Loader code is measured by the ROM code into PCR 1.
Similar(24)
There is a large variety of approaches for SDM using PCR [ 1, 3, 4].
Several studies have linked specific pathogens to ocular inflammatory syndromes using PCR [1].
HFE mutation was analyzed measuring fragment-length polymorphisms by PCR [1].
specific PCR [1] and a single nested PCR targeting the Internal Transcribed Spacer (ITS) regions of trypanosome ribosomal DNA [2].
For each PCR, 1 2 μL of this lysate was used.
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