Sentence examples for pbs 1 from inspiring English sources

They were then permeabilized (with PBS, 1% BSA, 0.1% Triton X-100, and 2% serum) for 5 min and saturated with the blocking buffer (PBS, 1% BSA, and 2% serum) for 30 min.

The SVF was then kept in FACS buffer (PBS, 1 mM EDTA, 3% HI-FBS) on ice.

After incubation, cells were harvested, washed twice in FACS buffer (PBS 1 ×, 2.5% FBS, 0.1% BSA, 0.05% NaN3) and finally resuspended in 500 μl of the same buffer.

To determine KatA surface exposure, bacteria were blocked with PBS 1% BSA and incubated with 1 μg rabbit-anti-KatA for 1 h on ice.

Cells were then washed twice in ice cold PBS and re-suspended in PBS (1 ml).

All antibodies were diluted in PBS 1% BSA.

Cells were harvested, washed once with PBS 1× and incubated with 10 μM (in PBS 1×) for 30 min at 37°C prior to analysis by flow cytometry.

Blocking was performed in PBS 1×, 0.2% Tween, 5% dry milk powder (Marvel).

Control group received 0.1 mL of PBS 1×.

Grids were blocked with PBS 1× + 5% BSA for 30 min, RT and washed with PBS 1× + 1% BSA + 0.1% Tween-20.

Cells were then washed 2 times in wash buffer (PBS +1% BSA and 0.02% NaN3).