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The maximal time allowed for paw removal was 30 s.
Oxotremorine also increased latency of back paw removal on the hot plate suggesting an enhanced antinociceptive response.
Animals were placed on the hot plate and we measured the time required for back paw removal before and 30 min after the injection of oxotremorine.
Variation in latency of back paw removal from the hot plate 30 min following the injection of oxotremorine (OXO; 0,1 and 0.25 mg/kg) in (a) post and (b) prepubertal NVH lesioned and sham control rats.
To perform the test, a radiant heat source was focused on the right hind paw, and withdrawal latency was determined as the time taken for paw removal.
The threshold was recorded as the force that elicited the hind paw removal reflex (the mean of three measurements performed at 1 min intervals).
Similar(54)
In prepubertal NVH rats, oxotremorine (0.25 mg/kg) failed to differentially affect back paw's removal (Figure 6b).
Behavioral tests of adhesive tape removal from the paw were performed for all animals always the day before the fMRI session, and were preceded by 3 training sessions before animals were submitted to MCA occlusion.
The end point was characterized by the removal of the paw followed by clear flinching movements.
The investigator was trained to apply the tip perpendicularly to the central area of the hindpaw, and the endpoint was characterized by the removal of the paw.
This is why we deducted 2 Paws in the Hair/ Stain Removal, & Scratch Resistance category.
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