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Paw grip endurance (PaGE) was measured twice per week starting at age 70 d using the modified hanging wire test [12], [13].
Whereas short-term CR hastened clinical onset and shortened lifespan in male, but not female, G93A mice, and hastened disease progression with no effect on paw grip endurance [11].
Forelimb grip strength as well as all paw grip strength was measured three times and the average value was taken.
Rats were trained for 2 days prior to the surgery on two motor tests: a variation of the PGE (paw grip endurance) task and the rotarod (Columbus Instruments).
Locomotor activity, motor strength, and motor coordination were evaluated as described in previous works [ 19, 28] by using stride length test, paw grip endurance (PaGE) test, and rotarod test, respectively.
This progression is shown in Figure 1 at days 1, 4 and 7. Quantitative assessment of these motor alterations in the rotarod and the paw grip endurance (PGE) test is shown in Figure 2, with emphasis in the values at these days to show the correlation with the position of the paws at these times.
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Four-paw grip strength was measured following the manufacturer's instructions.
In a first step, animals were monitored in vivo by 4-paw grip strength measurements and wire hanging tests.
The analysis of 1.5-year-old WT, HET, and HOM animals revealed a statistically significant reduction in the 4-paw grip strength in HOM mice as compared to WT mice (Fig. 7a).
He dangled overhead, upside-down, his tail and four paws gripping different branches.
The mice were held above the apparatus grid with their front paws (forelimb grip) or all four paws (all limb grip) grasping the grid, then pulled back by the tail following the axle of the sensor, horizontally and steadily, until they released the grid.
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