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Other common techniques include pausing, using rests, rapidly speeding up the BPM, and using progressing kicks from quarter- to eighth- to sixteenth-, note kicks, and so on.
The wild-type curve is fit to the one-dimensional diffusion model for backtracked pausing, using a γ u value of 0.6 (red dashed line).
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Hands-off intervals for rhythm analysis could be completely eliminated by assessing the rhythm during ventilation pauses using a high temporal-resolution SAA.
Given the uncertainty of safety and the common use, we performed a nationwide study of the relationship between SSRI use and congenital malformations with focus on congenital heart defects and comparison with paused use during pregnancy to account for special characteristics of women using antidepressants.
"We've set out the law clearly to Facebook, and we're pleased that they've agreed to pause using data from UK WhatsApp users for advertisements or product improvement purposes," she adds.
"So consider putting one of your services or subscriptions on pause using a vacation mode if they offer it, and see if you miss it after a month".
Lysed cells were sonicated for 3×30 sec on ice with 30 sec pauses using a probe sonicator (Labsonic-M, 3-mm probe; cycle 0.5, 30% power; Sartorius AG, Goettingen, Germany, http://www.sartorius.com) to produce ∼400 500 bp chromatin fragments.
We simulated transcriptional pauses using the lower bounds of kf1 and kb1 and obtained a correction factor of ∼7 for the mutant Pol II.
NPCB particles were suspended in 0.2 µm filtered, -irradiated Nanopure Diamond UV water (Pyrogens: < 0,001 EU/ml, total organic carbon: < 3.0 ppb [Jackson et al., 2012a, b]), and sonicated on ice, for 16 min without pause using a Branson Sonifier S-450D (Branson Ultrasonics Corp., Danbury, CT) equipped with a disruptor horn (model number 101 147-037).
After evaporation of the chloroform, the lipid films were dried under vacuum for ≥2 h, reconstituted in buffer A containing 20 mM BME to a concentration of 3 mM total lipid, and sonicated on ice 4 min (intervals of 1 s separated by 2 s pauses) using a Sonics Vibra-Cell probe sonicator to yield small unilamellar vesicles (SUVs).
Ron Kane, Cubic's New York spokesman, explains that the pause uses technology similar to that found in the sophisticated weapons systems his company has created for the Defense Department.
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