For recognition of conspecific calling songs, female T. viridissima assess the syllable-pause structure rather than the double-syllable rate [57].
We assayed the replication fork pausing structures at the chromosome 17 centromere by two dimensional neutral agarose gel electrophoresis.
The codon usage patterns also point to translation "pauses" between secondary structure elements, as happened with the ribosome occupancy profiles, possibly for allowing these to fold properly.
I can decide and delegate at leisure, clear my head, look inside myself, reconsider, and pause to analyze the structure of the situations I find myself in.
However, these structural changes have not been fully defined, nor is it understood how the CJ sequence itself causes the EC to change its structure, to pause, and to become less stable.
It is well known that translating ribosomes tend to pause at specific secondary structure motifs (such as hairpins and pseudoknots).
It is known that some mRNA structures, especially pseudoknots, can cause the translating ribosome to pause upstream to such structures [ 32– 32].
We have patented Translation Engineering™ technologies to manage the complexity of gene design to account for codon pair usage, translational pausing signals, RNA secondary structure and user-defined sequences such as restriction sites.
In the second experiment, it was showed that the distribution and structure of pauses in speech, represented by three parameters, are specific for the type of speech and sufficient to automatically classify them with 78%% accuracy.
However, sequences already transcribed can significantly alter the dynamics of pausing via the secondary structure of the nascent RNA.
Together, these data are consistent with a translational pausing mechanism whereby structured regions preceding the frameshift site regulate ribosome spacing.
