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The important point isn't the exact length of the sprint or the break, it's to figure out what "pulse and pause" cycle works best for you.
After denaturation, pause cycle and add 1 μl of Pfu.
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Additionally, patients randomly received MI-E in one of the sessions (one day intervention).MI-E treatment consisted in 4 series of 5 in-expiratory cycles at +/− 40 cmH2O pressure, 3 sec inspiratory time, 2 sec expiratory time, and 1 sec pause between cycle.
When proliferating cells encounter DNA damage, they must pause cell cycle progression in order to allow repair of the lesion(s), if possible [21].
To break the cycle, pause several times a day.
Cartilage (1 g each) was pulverized in a Spex Certiprep freezer mill Model 6750 (15 Hz; 2 × 1 min with 1 min pause between cycles) under liquid nitrogen for protein extraction and RNA preparation.
You sense in her a great affection for The IT Crowd but a restlessness, too, with the gag-pause-repeat cycle of sitcom work.
Mr. Hiller and Mr. Stanton said they hoped that the latest newsroom downsizing would be the last for a long while, pausing the cycle of cut after cut that has made it hard for people to catch their breath and look to the future.
This week's special question asked AAII members for their thoughts on the prospect of the Federal Reserve pausing its cycle of raising interest rates.
She repeated the ol' type-pause-type cycle a few times, before looking up to ask me, "What was the title again?" "Love Wins.
Cells were then washed in PBS, resuspended in 200 µl of ChIP lysis buffer [1% sodium dodecyl sulfate (SDS), 10 mM EDTA, 50 mM Tris-HCl (pH 8.0), and protease inhibitors] and sonicated (60% maximum output for 15 s with 3 min pause, 3 cycles; Branson Sonifier Type 450 with microtip; Danbury, CT) in an ice bath.
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