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A fast disappearance of pause bands would unambiguously show that the corresponding complexes have backtracked.
To assign precise pause positions, we also compared pause bands to 3′-deoxyNTP-generated ladders using shorter templates and high-resolution gels.
Our data already show that GreB causes H NS stimulated pause bands to disappear (e.g., the C346 pause in Figure 6).
It is unclear to us why immobilizing the complexes, trapping them at the site by washing and NTP deprivation, and then showing that addition of GreB causes the pause bands to disappear provides new information.
Indeed, showing the GreB suppression of the pause bands occurs on the time-scale of transcription is a more relevant result than showing that backtracking occurs in halted complexes.
Similar(55)
Pausing is quantified by dividing the signal in the ∼35-nt pause RNA band by the sum of this signal and the signal in the terminated (full-length) band; this ratio is expressed as a percentage (relative abundance).
As Ms. Jones engaged less in singing than in dramatic reading, with overenunciated syllables and tightly clustered sprays of words separated by long pauses, her band sounded cowed.
During a pause while the band tuned up, one heckler shouted "Judas!," as if Dylan was both apostle and traitor to his true disciples.
When Jay-Z left, there was only a split-second pause before the band tumbled into "Chalk Dust Torture," with Mr. Anastasio skipping nimbly through the lyrics -- a subtle nod, perhaps, to Jay-Z's rhyme style.
In April the same year, manager Martin Pike announced a pause in the band's career together for the time being.
Life in Burns, a two-lane highway town that has never entirely crawled out from under the closure of the timber mills, has been put on pause since a band of armed ranchers and activists broke into a compound of vacant government buildings on the edge of a wildlife reserve outside of town.
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