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As this particular passage from Just Kids set my mind's light bulbs aflashin', giving me cause for pause at which time thinking ensued (another rare occurrence), I'd thought I'd share it with you.
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If user-interactive components are present in a workflow, the processing of the workflow pauses at which point the user is expected to provide some sort of input, which in most cases will be manual annotation.
Bridged H-NS filaments slow RNAP at a subset of pause sites at which RNAP appears susceptible to backtracking, as evidenced by the large effect of GreB in suppressing H-NS stimulation of pausing at these sites, by the presence of short U-tracts just upstream from the pause sites at which H-NS has the largest effects, and by direct detection of backtracking for the C346 H-NS stimulated pause.
The model just decides whether to increase or decrease the latency of PC pause by comparing the current timing of the pause and the timing at which the PF-CF coupling occurs.
If you wish to indicate a pause or trailing off: Choose the point at which the pause or trailing off occurs.
This mechanism should not be confused with the phenomenon of genome-wide RNAPII pausing at promoters, which represents an intermediate step between RNAPII initiation and elongation phases and might be a common mechanism regulating differential gene expression in metazoans [ 84, 85].
(B ) Visual scheme of the stage at which pause occurs during translation.
(E ) Visual scheme of the stage at which pause occurs during translation, similar to Figure 2B.
(D ) Visual scheme of the stage at which pause occurs during translation in B. subtilis, similar to Figure 2B.
Spike experiments at 29 °C were paused after 25 min at which point solutions containing DNA, theophylline, or controls were added to the appropriate wells, and then placed back on the plate reader for fluorescence monitoring.
He has become so immersed in his work, he said, that it has been hard to find a point at which to pause and pull it all together.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com