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A strategy for patterned cell adhesion based on chemical surface modification is presented.
Masked irradiations open a simple, fast (cell patterns are obtained within a few hours), and economical route to obtain chemically patterned cell culture substrates.
Taking advantage of this unique property of cross-linked albumin, the authors fabricated patterned cell co-cultures with desired patterns and cell types.
Our approach could be used towards the fabrication of "designer" substrates or devices that not only allow patterned cell growth, but also permit dynamic cell repositioning.
Furthermore, we confirmed that patterned cell viability does not depend on the target cell concentration in the reservoir (see Fig. S4 online).
Arabidopsis qrt mutants produce tetrads of fused pollen grains, each with a normally patterned cell surface [ 9, 10].
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To address this, we incorporated UV crosslinking ability in chitosan, allowing fabrication of patterned cell-laden and rapid transdermal curing hydrogel in vivo.
N-MAC would allow rapid, robust and cost-effective fabrication of patterned cell-laden polysaccharide microgels with unique amino groups serving as building blocks for tissue engineering and rapid transdermal curing hydrogel in vivo for localized and sustained protein delivery.
Anisotropic patterns with water wettability difference can be useful for patterning cells and other materials using their selective growth on the hydrophilic part of the pattern.
We report an effective approach to patterning cells on gold silicon dioxide substrates with high precision, selectivity, stability, and reproducibility.
Excess cells are then washed away (Figure 5A), and the chip can be flipped over to transfer the patterned cells onto any desired substrate.
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