This pattern largely correlates with endogenous mpz expression during zebrafish development—mpz is expressed in a dynamic spatial and temporal fashion from early stages in the lateral tegmentum, lens vesicles, optic nerves, hindbrain and anterior spinal cord [22], [23], [24].
We had investigated the expression of such TSPY-transgene in the LADY mouse model of prostate cancer, harboring a SV40 T antigen gene directed by a rat probasin promoter; and compared the expression pattern with those of endogenous TSPY gene and biomarkers in human prostate cancer specimens.
We also found a similar pattern with antibodies to the endogenous proteins (not shown) and so these findings agree with the comparative proteomics.
In line with endogenous staining patterns, overexpression of TDP-43 causes a predominant nuclear localization [ 99, 130, 176, 199].
This fractionation pattern is equivalent to that seen with endogenous L22 (lower band) in these cells.
This fractionation pattern is distinct from that seen with endogenous L22 and wild-type GFP-L22 (Fig. 6B, fractions 9 12, top panel) in that unlike these proteins, m88 did not co-fractionate with 28S rRNA (found in fractions 9 12) and was not incorporated into ribosomal subunits.
The overall pattern of CD68 was in congruence with the pattern of endogenous TPEF that was acquired before staining.
The β-gal activity programmed from the GT correlated tightly with the pattern of endogenous Rest mRNA.
The most prominent expression domain of a transgene under the regulation of MmURE2 or DrURE2 has been shown to be the forebrain, more precisely in the ventral telencephalon and diencephalon, in agreement with the pattern of endogenous expression of Dlx1 and Dlx2 genes [ 17].
Previous work has shown that PER::luc activity correlates with endogenous PER2 expression patterns in the SCN and peripheral tissues and allows tracking of PER2 oscillation dynamics in real-time (25, 33).
