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Based on the overarching structural pattern, we determined the diameter of the eroded Oasis structure at 15.6 ± 0.5 km.
To quantify the growth pattern, we determined the wet weight for six male individuals in each age group.
For the analysis of the global NBPF expression pattern, we determined relative gene expression levels using an optimized two-step SYBR Green I RT-PCR assay employing several reference genes [19].
For analysis of the NBPF1 expression pattern we determined the relative gene expression levels by an optimized two-step SYBR Green I RT-PCR assay using the two most stable housekeeping genes in neuroblastoma cell lines [19].
As DNMTs play a crucial role in the regulation of DNA methylation pattern, we determined the expression profiles of DNMT1, DNMT3a, and DNMT3b.
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For each pattern we determine its importance.
From the observed staining patterns, we determined that when fractional mitochondrial or lysosomal mass accumulation was ≥40% there was an almost equal probability that the images were scored as being localized to (or not localized to) mitochondria or lysosomes, irrespective of the predicted percent accumulation (Figure 5A, B).
To study the nucleotide substitution patterns we determined the relative rate of nonsynonymous substitutions for all Ymf and KPC genes using T.paravorax sequences as outgroup.
We first evaluated the associations and distributions separately for the HLA-B*57, HLand5' and HCP5 alleles (Figure 3), and then because of their LD patterns, we determined the associations for B*57-containing genotypes that contained or lacked HLand5' and HCP5 alleles (Figure 4).
To further show that abnormal Bcd can lead to normal Eve patterning, we determined the intensity profile of Bcd and Eve in immunostained wild-type embryos that were placed in a microfluidic device exposed to a temperature step approximately 20 minutes after fertilization.
To gain a deeper understanding of the localization patterns, we determined the subcellular localization of TLPs.
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