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The second experimental setup compared the methylation pattern of transgenic regions on single and stacked transgenic events in the same genetic background (DKB240).
Here we analyze the pattern of transgenic expression of normal (TTRwt) and mutated (TTR-A) human TTR and pay particular attention to the ultrastructure of relevant tissues.
To rule out that STZ administration may have altered expression level or pattern of transgenic tau, we performed immunohistochemistry of brain sections and Western blotting of brain extracts, using phosphorylation-independent tau specific antibodies, HT7 and TAU5.
Immunofluorescence using anti-MeCP2 and anti-GFP antibodies on midsagittal sections of the whole brain shows that the distribution pattern of transgenic MeCP2 parallels that of endogenous MeCP2.
The expression pattern of transgenic worms was monitored by mounting sodium azide- or levamizole-treated animals on 2% agarose pads, on glass microscope slides.
The hippocampal region of the brain from 90-week HGPS animals was analyzed for the expression pattern of transgenic lamin A/progerin in combination with mouse lamin A/C (Fig. 1A F).
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Additional file 7: GUS gene expression patterns of transgenic lines carrying a URS:: GUS cassette shown by qRT-PCR analysis.
Through Q-PCR analysis, we found that both the pips_m and pips_h cell lines expressed Oct4 and c-Myc transgenes (Oct4_v and c-Myc_v), but the expression pattern of the transgenic Sox2 and Klf4 (Sox2_v and Klf4_v) were different between the pips_m and pips_h cell lines.
The GUS staining pattern of the transgenic plants harboring the RAP2.4 promoter construct was similar to that of the plants harboring the RAP2.4L promoter construct.
Thus, overall the spontaneous breathing pattern of male transgenic UBB+1 mice was affected by a statistically significant increase of the expiratory time over age.
The branching pattern of a transgenic line that constitutively expresses the Arabidopsis cytokinin degradation gene, CYTOKININ OXIDASE-2 (CKX2oe) (von Schwartzenberg et al., 2007), was quantified and found to be similar to the model output in having fewer branches and a larger apical inhibition zone.
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