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The expression pattern of transcripts encoded by TaSSIVb was then determined by semi-quantitative comparative reverse transcription (RT -PCR.
SR45 also changed the splicing pattern of transcripts from several other SR genes.
The effectiveness of the bar-coding was indicated by the distinctive pattern of transcripts found in each tissue and the limited distribution of some transcripts, e.g. vdg3 only in digestive gland, foot proteins isolated to that tissue, etc.
This point can be further illustrated by Figure 3 which shows expression pattern of transcripts called "Absent" and "Present" in the contexts of a biological pathway (a fragment of insulin signaling pathway, data from Metacore database, GeneGo Inc).
The sixth candidate, Act5C, had several homologs that were highly conserved at the nucleotide level, raising the possibility that several individual genes likely contributed to the pattern of transcripts observed in Fig. 2. Thus, this candidate was not characterized further at this time.
Furthermore, the alternative splicing pattern of transcripts of several other SR genes was altered in a mutant, sr45-1, suggesthat thet the observed phenotypic abnormalities in sr45-1 are likely due to altered levels of SR protein isoforms, which in turn modulate splicing of other pre-mRNAs.
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Additionally, our investigations revealed an intricate pattern of transcript overlapping, which might produce transcriptional interference between the transcriptional machineries of adjacent genes, and thereby may potentially play a role in the regulation of gene expression in circoviruses.
Interestingly, detailed analysis of transcription during intraerythrocytic development suggests a temporal pattern of transcript accumulation in the early trophozoite stages when the parasite becomes more metabolically active.
We also found a pattern of transcript levels that would be consistent with a relatively normal differentiation outcome but increased proliferation by the bone marrow macrophages that were assayed after 14 days of space flight.
However, the diurnal pattern of transcript and metabolite levels within the rice Fe deficiency response has not been as extensively investigated as the Fe deficiency response of barley.
We also found an unexpectedly dynamic pattern of transcript isoform regulation, suggesting a critical regulatory role during HSC differentiation, and a cell cycle/DNA repair signature associated with multipotency in MPP2 cells.
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