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In each system, a set of nine internal control samples with a unique pattern of probe reactivity was included among the samples investigated.
Spoligotype analysis showed seven isolates with the typical Beijing/W pattern of probe hybridization only to spacer sequences 35 43.
Figure 3 illustrates how the pattern of probe binding may be used to characterize 'regions of difference'.
If the sequence difference is a single-base polymorphism, the pattern of probe intensities at that position will be discernibly different than expected.
Five males and 5 female control DNA samples were used to establish the relative pattern of probe amplification in normal samples.
Ten DNA samples extracted from healthy individuals were first analyzed to assess the pattern of probe amplification in normal tissue, to create a mean "reference control" pattern, and to exclude the possibility of copy number polymorphism.
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In general, four different patterns of probe set responses are visualized in the heat map.
By hybridizing genomic DNA from several bacterial species, the authors demonstrated that their array produced reproducible patterns of probe intensities (or 'signatures') that distinguished between one species and another.
Interestingly, for five of these specimens, large clonal populations of cells showed gain of chr3, 5, and 17 (probe sets 2 and 3) but none showed loss of 3p25 (VHL) or 3p, scored relative to 3q11 in probe set 1. Examination of the scoring patterns of probe set 1 for these five specimens revealed no gain of 3p25, 3p21, or 3q11 loci, distinct from that found in probe set 3 for the 3q26 locus.
The data for each sample are presented as a matrix, where each column is one of the probes and each row is a 'cell count pattern' of four probe counts, such as 2,3,4,1 (or eight counts for breast cancer), and the number of cells matching that pattern.
This is also the first single crystal structure which reveals directly the coordination pattern of fluorescent probe bearing di-2-picoly-amine unit with Cu II).
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