Utilizing high-throughput screens, we identify prion-curing mutants and engineer "anti-prion drives" that reverse the non-Mendelian inheritance pattern of prions and eliminate them from yeast populations.
The lesion profile in the brains of the BSE-challenged fish shares both similarities and differences in comparison to this histological and immunohistochemical pattern of mammalian prion diseases.
The quantity and the pattern of cortical prion pathology was recorded for all cases as shown in Table 2.> -wrap-foot>> -wrap-foot> Scoring scheme used to semiquantitatively assess the frequency and density of granular or filamentous inclusions in DAB stained paraffin sections on a LEICA DM2500 with a 40× HCX Pan Apochromat objective.
Sporadic, inherited and acquired prion diseases show distinct histological patterns of abnormal prion protein (PrP) deposits.
The extent of neuropil vacuolation and pattern and intensity of prion protein deposition was assessed semiquantitatively.
The increased amounts of PrPd demonstrated in a reactive compared to non-reactive lymph node, and in chronic inflammatory tissue in the lung, show that the state of activity of the lymphoreticular system has an influence on the pattern of distribution of prion replication/accumulation in lymphoid tissue.
In mice, sheep and deer, chronic lymphocytic inflammation can shift the distribution pattern of PrPSc or prion infectivity to non-lymphoid organs, being they in the kidney of mouse and deer [23], [24], liver and pancreas of mouse [25], and mammary gland of sheep [8].
In 1998, a new type of scrapie called scrapie Nor98 was detected [ 2] and in 2005 the European Food Safety Authority (EFSA) defined diagnostic criteria for classical scrapie (CS) and for atypical scrapie (AS), including Nor98, based on the results of Western blot pattern of the pathogenic prion protein (PrPRes) [ 3].
Sections of paraformaldehyde-fixed, paraffin-embedded human cerebellar tissue samples from a patient with sporadic CJD (sCJD) with kuru plaques and synaptic immunohistochemical type of prion deposition pattern were used in the study.
Collinge's team found that the prion pattern of vCJD--which has stricken victims up to age 45--resembles the BSE pattern far more closely than that of people suffering from classical CJD, a more common, albeit rare, condition that tends to strike older people.
