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Similar to LEIO, MFH-LEIO did not demonstrate any specific pattern of pathway activation compared to the rest of the samples.
However, non-responders showed a qualitatively similar dynamic pattern of pathway activity compared to age-matched responders.
These results indicated strong similarity between cell lines and tumours in the pattern of pathway enrichment (Fig 2).
Surprisingly we have found that the pattern of pathway utilisation in normal melanocytes was not distinct from those exhibited by the melanoma lines in the presence of serum.
We believe that our studies could guide the development of prospective clinical studies that would evaluate the efficacy of therapeutic regimens, based on a combined pattern of pathway and chemosensitivity within a given subtype.
Taken together, the results indicate that all the analyzed human cells with de novo created trisomies and tetrasomies elicit a nearly identical pattern of pathway changes regardless of the identity of the supernumerary chromosome and the cell line.
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Our analysis identified a specific pattern of pathways that are altered in all compared model trisomic and tetrasomic cell lines (Table 1).
Patterns of pathway deregulation in 211 breast tumor samples arising in women aged ≥65 years were evaluated.
As shown in Figure 7, different patterns of pathway activation were associated with resistance to different chemotherapy agents.
Using the previously described signatures of oncogenic pathway deregulation, patterns of pathway deregulation in 200 breast tumor samples arising in young women aged ≤45 years were evaluated.
Given the comparable patterns of pathway activation and corresponding prognosis despite ER classification, we conclude that the patterns of oncogenic pathway deregulation among breast tumors arising in young women in this analysis are independent of ER status.
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