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We further investigated the protein expression pattern of full length Notch 1 (330–220 kDa), the Notch 1 extracellular domain (Notch 1-EC, 120 kDa) and of the downstream transcription factor HES1 (32 kDa) in 28 of the 32 ovarian tumours (16 ovarian carcinomas, three borderline tumours, nine ovarian adenomas), in three human ovarian cancer cell lines and one ovarian surface epithelium cell line.
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We next looked at the expression pattern of full-length MUC1 versus MUC1* healthy and cancerous human tissue specimens from untreated patients.
Instead of fewer and larger speckles with full-length SR45 protein, there were numerous smaller speckles with the RS1 or RS2 domains spread all over the nucleus (constructs Δ99 414 and Δ1 172, Figure 5B), which resembled the pattern of full-length U1-70K (Figure 1).
The expression pattern of full-length ShCYC-B promoter as determined by GUS activity (Fig. 7B) was similar to that of the expression pattern observed by RT-PCR analysis in S. habrochaites (Fig. 1B).
To identify cells expressing EAT-4, we observed the expression pattern of full-length eat-4∷gfp in wild-type animals, and also in unc-104 e1265) unc-104 e1265tive in UNC-104/KIF1A kinesin-like mutantsrotein, to prevent the EAT-4∷GFP-causedefectivefluorescence of the nerve ring. UNC-104/KIF1A UNC-104/KIF1A
To examine intrafamily diversity pattern for each family, we performed network analyses on the basis of the alignment of full length sequences.
The binding patterns of full-length DevR and DevRC proteins were compared in EMSA and DNase I footprinting experiments.
A CNGC19FL-mCherry fusion was generated to directly compare the subcellular localization patterns of full-length CNGC19 and CNGC20 within the same cell.
Further analysis showed that these binding events were virtually superimposable on the binding pattern of the full length LARS2 (Fig 3D), consistent with suppressive effect seen here and previously in yeast (Francisci et al, 2011).
Notably, the pattern of expression of full-length MUC1 is clustered at one or two points on the cell surface, which is reminiscent of the tendency of MUC1 on healthy epithelium to cluster at the apical border.
In summary, the two S511A/S511D and T1471A/T1471D pairs manifested very different CFTR cleavage patterns and abundance of full-length CFTR when present on a wt background.
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