In comparison with the controls, the hypothyroid boy showed a marked increase in the total AUC of prednisone (3360 μg h/L versus 215 ± 83 μg h/L) and prednisolone (4040 μg h/L versus 724 ± 77 μg h/L), and an altered pattern of endogenous cortisol, which is known to be impaired in hypothyroid subjects.
Also, in the gel shift assay, the pattern of endogenous NFkappaB binding to its probe was not changed in any manner by Pim-1.
Expression of the dominant-negative E-cadherin construct H-2Kd-E-cad caused a dramatic change in the pattern of endogenous E-cadherin localisation.
In immunofluorescence, pAb S1337 recognized exogenously expressed Kaiso, whereas the staining pattern of endogenous Kaiso in centrosomes was similar to that obtained with the monoclonal antibodies (see below).
In fact, the expression pattern of endogenous Dkk4 during normal development correlates inversely with secondary hair follicle formation [13], [19], [20].
Although the activity of recombinant IL-33 has been well characterized, little is known yet about the expression pattern of endogenous IL-33 in vivo.
In general, human LRRK2 expression does not influence the expression level or pattern of endogenous LRRK2 mRNA in the brain, spleen or kidney of transgenic mice (Figure S3).
To further study the Aβ binding pattern of endogenous anti-Aβ antibodies having the IgM isotype, we have screened a set of plasma samples from healthy donors.
The pattern of endogenous JIP1 protein overlapped in part with the VRK2A signal that has been reported to be bound to the endoplasmic reticulum by its C-terminal region (Fig. 5C, left column).
Reduction of either PUF60 or U2AF65 in cells alters the splicing pattern of endogenous transcripts, consistent with the idea that regulation of PUF60 and U2AF65 levels can dictate alternative splicing patterns.
It is clear that the ectopic expression pattern shown by the GFP-tagged SU VAR 3-9 does not always reflect the pattern of endogenous SU VAR 3-9.
