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Some previous studies (Zmasek and Godzik, 2011) analyzed the evolution pattern of domain repertoire in eukaryotes.
The non-uniform pattern of domain scores across the three phases suggested that respondents judged each phase separately as was intended by the questionnaire design.
We note that the majority of the viral FSFs originated either very early or very late, showing a clear bimodal pattern of domain appearance (red circles in the tree of Figure 1B and timelines of Figure 1C).
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Remarkably, Ethanol+Vinpocetine treated animals exhibited well defined and high-contrast differential maps with normal spacing and pattern of domains at both cardinal and oblique orientations, similar to maps observed in Saline treated animals (Figure 1a, differential maps).
This same procedure has been extended to the whole high-resolution map, thus obtaining the complete two-dimensional pattern of domains in Fig. 5 ▸.
The results are in Fig. 3 ▸, and the alternating pattern of domains is clearly visible (we note that the patterns measured at the two photon energies are shifted slightly due to the microfocused beam not being aligned to the centre of rotation).
These HS chain populations are then evaluated for structural patterns of domain organization.
The generated chains are transformed into strings of user-defined domains and examined for patterns of domain organization.
This model establishes the conceptual framework for a new class of computational tools to use to assess patterns of domain organization within glycosaminoglycans.
For a more in-depth analysis of the overall patterns of domain organization that characterize these chains, two analytical methods were applied to the output.
To address these challenges, we have devised a computational approach to predict fine structure and patterns of domain organization of the specific glycosaminoglycan, heparan sulfate (HS).
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