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Groups 2, 3, and 4 had a very similar pattern of deletion distribution, with the majority located near or at the common deletion breakpoint (8470 13447 nt).
The youngest patient group (<6 years old) showed a diffused pattern of deletion distribution in size and locations, with a significantly lower sequence homology flanking the deletion, and the highest percentage of deletion mutant heteroplasmy.
One of the challenges inherent in using the Cre/loxP system for conditional gene modification is to verify the actual pattern of deletion of the gene of interest.
The distribution pattern of deletion sizes in Kasalath against Nipponbare displayed two peaks if deletions of <1 kb were ignored (Fig. 2 and Supplementary Fig. S2).
Higher levels of mtDNA deletions were detected in postmitotic tissues e.g., skeletal muscle, heart and brain in contrast to kidney, spleen, skin and liver that are composed of dividing cells, indicating a tissue-specific "pattern" of deletion accumulation [ 7].
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And why the bizarre pattern of deletions and repostings surrounding some of the public documents supporting the allegation?
As the embryo developed, the CRISPR system repeatedly disrupted the target DNA in each cell, marking it with a pattern of deletions and insertions a distinctive barcode.
The older age groups showed rather discrete pattern of deletions with 44% of all patients over 6 years old carrying the most common 5 kb mtDNA deletion, which was found mostly in muscle specimens (22/41).
As seen in Figure 3, some of the strains within an ST-complex had a significantly different pattern of deletions throughout their pspA gene sequence when compared to the remainder of the strains within the specific ST-complex.
RT-PCR expression pattern paralleled the pattern of deletions.
Here we further verify the frequency and pattern of deletions at 14q in mesothelioma.
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