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Studying these cells using flow cytometry revealed a pattern of cell surface expression that was seen in breast cancer stem cells (CD44High/CD24Low).
We report here a targeting method that exploits the expression pattern of cell surface proteases to induce gene delivery to specific tissues.
Based on this pattern of cell surface marker expression, this subset has phenotypic characteristics typical of neutrophils [23].
We have previously characterised the promiscuous expression pattern of cell surface proteins on mouse embryonic stem (mES) cells.
Therefore, exploring the pattern of cell surface protein expression on ES cells is important for understanding the mechanisms of ES cell self-renewal and differentiation and can help to establish strategies for surface marker discovery.
Taken together, these results suggest that breast cancer cell transendothelial migration is closely correlated to both the expression level and localization pattern of cell surface E-selectin binding protein(s).
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We found that CD158k+ T cells and circulating CD4+ T cells from MF patients exhibited unexpected patterns of cell surface expression with a marked heterogeneity of circulating lymphocytes even at initial diagnosis.
These findings are consistent with the expression patterns of cell surface markers during normal human erythroid differentiation [ 22, 23].
The expression patterns of cell surface antigens were then compared between the treated and untreated cells using the LyoPlate cell surface antigen array (BD Biosciences, San Jose, CA, USA).
The patterns of cell surface expression of uPAR and β1 integrin subunit on HCT116 clones were assessed by flow cytometry using anti-uPAR and anti- β1 integrin antibody.
It was used to modify the glycosylation patterns of cell surface glycoconjugates during thymocyte selection processes during postnatal period in mice (Balcan et al. 2008).
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