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The pattern of band changes drastically when we used the extract from cells producing Pol ι.
Each polymorphism would be expected to give a different pattern of band changes.
More strikingly, when the experimental difference spectrum is compared to a model spectrum created by subtracting the spectra of poly A from dGMP, the agreement between the experimental and predicted pattern of band intensity changes is excellent.
The ChIP DNA samples were analyzed via quantitative PCR using the ChIP primers for Ch25h "region 11" and values obtained reflect the pattern of band intensities on the ChIP gel (Table 2).
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The resulting images of photographed face next to painted face, with a percentage score indicating how good the match was, seemed vaguely diagnostic, as if the painting had materialized like the pattern of bands in a DNA test.
A ladder-like pattern of bands, specific for F. piscicida, was amplified from positive samples.
The RT-LAMP product had a ladder-like pattern of bands and the optimal reaction condition for this assay was determined to be 40 min at 63 °C.
Our results show that the ladder-like pattern of bands sizes from 234 bp specifically to the E. ictaluri gene was amplified.
The LAMP products had a ladder-like pattern of bands from 188 bp when electrophoresed on an agarose gel and its specificity was confirmed by digestion with Hinc II enzyme.
By the detection system, 241 bp target DNA was amplified and visualized as ladder-like pattern of bands on agarose gel within 60 min under isothermal conditions at 65 °C.
A ladder pattern of bands after gel electrophoresis was observed for only BPV isolates and showed that the BPV LAMP assay was highly specific without any cross-reactivity with other related viruses.
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