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Cigarette smoke-exposed populations have been successfully distinguished from control nonexposed populations based on the expression pattern of a subset of genes, thereby demonstrating the utility of this approach in identifying biomarkers of exposure and susceptibility.
To quantitatively confirm these results, the predicted localization pattern of a subset of probes showing characteristic staining pattern was independently scored by an expert observer that was blind to the chemical structure and the predicted localization of the probes (Figure 5).
We therefore sought to analyze the intracellular distribution pattern of a subset of our crossreactive DUBs, using confocal microscopy.
Using different methods such as semi-quantitative and quantitative-PCR we confirmed the expression pattern of a subset of genes included in our list (Figure S2).
Based on the spindle-shaped localization pattern of a subset of the Qdots, along with their directionally biased movement, we speculated that some actin filaments could be traveling down the microtubules of the central spindle toward the contractile ring.
The distinctive expression pattern of a subset of genes was validated by quantitative reverse transcription PCR.
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On the other hand, gene-module based methods take advantages of biclustering algorithms [ 7, 8] or item-set mining algorithms [ 9] to detect a cluster of genes which share similar patterns on a subset of gene conditions.
We have also compared the average likelihoods of the phonemes and phoneme error rate to find out if the drop in ASR performance with ageing is due to changes in articulation patterns of a subset of the phonemes.
As additional validation for the functionality of the observed Ubx binding sites, we extended previous expression analyses [12], [13], [14], [15], [17], [26], [27] and compared the expression patterns of a subset of Ubx-bound genes in the wild type haltere and wing imaginal discs, which are Ubx-expressing and –nonexpressing tissues, respectively.
Gene expression patterns of a subset of genes were validated using real time PCR.
Real-time PCR analysis confirmed the differential expression patterns of a subset of these genes.
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Justyna Jupowicz-Kozak
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